Hansen Iron Trigematein Method

Hansen Iron Trigematein Method: Description and Application

The Hansen iron-trigematein method, also known as the Hansen method, is a tissue staining method developed by the Danish histologist F. S. Hansen in the early 20th century. This method is used to detect iron in tissues by staining it with trihematein compounds.

The principle of the method is that trigematein forms stable compounds with iron, which then turn blue-violet. This makes it possible to accurately determine the location and amount of iron in tissues, which is of great importance for the diagnosis of various diseases such as hemochromatosis and anemia.

The application of the Hansen method in medicine is especially important for identifying and diagnosing various diseases of the blood and organs, such as the liver and spleen. In addition, this method can be used to study glandular organs in the field of animal science and biology.

Hansen staining uses fresh or fixed tissues, which are then treated with various reagents, including trihematein and acid solutions. After staining, the tissues are thoroughly washed and can then be examined under a microscope.

Although the Hansen method is not the only method for staining iron in tissue, it is still widely used in medicine and science due to its accuracy and reliability. This method can also be applied to detect other elements such as copper and nickel in tissue, making it a versatile tool for many studies.

In conclusion, the Hansen method is an important tool for detecting iron in tissues and has wide applications in medicine and science. It helps diagnose various diseases and can be used to examine various glandular organs. Because of its accuracy and reliability, the Hansen method remains an important staining method in our modern medicine and scientific community.

The ancient sages called for enjoying the process of life without holding a grudge against those who attack you. In addition, you should not remain indifferent while watching someone who needs your help suffer. Hansen in his work describes a method that will help restore balance. And at first glance, all you need to do for this is take an iron tablet. Still, he recommends taking a few simple steps beyond that: maintaining your mood, seeking help from people and spending more time outdoors, taking care of your body and staying stable. The method includes relaxation and avoiding food that can cause gas. This is a time of deep inner rest and self-contemplation, which allows you to overcome stress, relieve tension, fatigue, anxiety and restlessness. Helps you learn to capture moments of joy and benefit from them for yourself and others. Enjoying a leisurely walk through the streets, enjoying the amazing miracle of nature, we discover the reserves and harmony of the soul. The ability to balance and direct your attention to maintain peace of mind becomes an important life skill. Thanks to this, a person acquires the ability to survive in unfavorable external conditions and maintain peace of mind despite weather conditions or unforeseen circumstances. Those who are not accustomed to a constant relaxation regime need an adjustment period. Hansen describes the following chain of events, each of which will help a person realize the importance of balance: • Ingested material is absorbed best by a person when digestion is in balance. Lunch can be called a balance of the body, and not an end in itself. It will not make you feel good if its level is different from the level of hunger and thirst. When the body is supplied with carbohydrates and proteins, it is easier for it to produce energy for activity. A lack of potassium and an excess of phosphorus can disrupt the functioning of the heart. If essential vitamins are missing, resistance to infections decreases. Poor diet leads to sleep disturbances. Eating a meal and a glass of water at noon is designed to overcome symptoms and prevent illness. The stomach craves something to eat every hour. What you should abstain from is a large amount of dry, hard bread or a tight sitting position. The body tends to deny stressful situations and, as a result, aggravate internal disorders. Diet has a strong influence on the state of the nervous system. Physical inactivity - insufficient movement, lack of physical activity leads to blood stagnation and lowers blood pressure. Circulatory disorders can negatively affect the brain. It is worth taking care that Give your body more room to move. By maintaining a connection with the earth, a person improves his nervous system, increases the level of triglycerides, cholesterol and estrogens in the blood. After a good awakening and a solid breakfast, the body needs 15 minutes for the blood flow to activate. A boost of energy appears as soon as the brain begins to function. The brain literally works at high voltage: the level of ventilation of the lungs, heart rate and even blood pressure will increase. A person avoids the desire to drink tea. Even the feeling of vigor requires the presence of air. After finishing drinking tea, you should leave your spirit free. Otherwise otherwise

**Hansen iron-trihemateene method** is one of the classical methods of staining tissues for microscopy. This method was developed by F.S. Hansen in 1807 and is still widely used in histological studies of various organisms.

This method is based on the oxidation of tissue proteins in an alkaline environment with iron(II) trihematecate [1-3]. Hansen called it the iron-triquemate method, but later, due to an error in the description, he lost the authorship of this method.

The principle of coloring using this method is the binding of metal ions by iron-containing tissue components, which transforms them into a colored state. As a result, the color of the tissue appears as a purple-blue color, and structures not affected by the dye remain transparent.

The steps of the Hansen stain technique include:

Sample preparation. Before dyeing, the fabric must be placed in a heated alkaline environment (sodium or potassium hydroxide solution) or left for some time in a saline solution to destroy acidic components that may interfere with the oxidation reaction. Dye treatment. Coloring occurs when an alkaline solution of iron(III) trikematecate is added to the tissue. In this case, the reaction occurs: Fe2+/Fe3+ + H2O = FeO42-/Fe2+ + 3OH-. This leads to the formation of the iron-coloring substance Turgen blue, which is capable of absorbing rays of different wavelengths due to the transition of an electron between the excited and ground states of the molecule. Fixation. After dyeing, the fabrics are fixed with an acetic acid solution to remove unabsorbed dye molecules and/or prolong dye fastness. Drying. At this stage, the tissue is washed with sodium carbonate solution to remove trikematetate ions and dried. Transparent background. The dyed fabric is washed with water and dried and transferred to filter paper. Thus, the Hansen ferritin staining method represented a significant advance in the development of methods for staining and visualizing macromolecular structures and microbial cells. It has long remained an indispensable tool for histological practice, allowing physicians and scientific researchers to identify diverse groups of cells and distinguish between different tissue structures. Despite the fact that Hansen ferritin is no longer the main method for staining tissue preparations, its knowledge and application are now very important in both scientific and applied fields of medicine for studying the macromorphological characteristics of tissues and determining the results of treatment of diseases.

Hansen's Glandular Trihematoin method is one of the most well-known methods of staining tissue for microscopic studies. This method was proposed in 1909 by the Danish histologist Hermann Adolf von Hansen (1856-1925) and is still one of the most popular tissue staining methods.

It is based on three substances: tetyachidine, hemarinic acid and dichromic acid osmite. These substances can be combined into one combination. It is called ferrous trihematoquinic acid because it turns the tissue dark blue. By the way, the method got its name from the author’s surname.

Before starting the staining process, it is necessary to prepare the histological samples that will be used for research.