The guaiac test is a blood detection method based on the change in color of a solution due to the interaction of hydrogen peroxide and guaiac resin. This method is used to determine the presence of blood in various fluids such as urine, stomach acid, vomit and feces.
Guaiac gum is an organic compound that contains guaiacol. When guaiacol reacts with hydrogen peroxide, a blue color is formed, which indicates the presence of blood pigments in the liquid. If blood is present in the liquid, the guaiac test will give a positive result.
Application of guaiac test:
– Detection of blood in the urine. The guaiac test is used in medicine to diagnose kidney and urinary tract diseases. If there is blood in the urine, the guaiac test gives a positive result.
– Diagnosis of gastrointestinal bleeding. The guaiac test can be used to diagnose gastrointestinal bleeding caused by various diseases.
– Monitoring the condition of the blood. The guaiac test can be used to monitor blood status in patients who are on hemodialysis or receiving blood transfusions.
Advantages of guaiac test:
– Speed and ease of implementation. The guaiac test is quick and easy to perform, allowing you to quickly determine the presence of blood in a liquid.
– High sensitivity. The guaiac test is highly sensitive, allowing it to detect even small amounts of blood in a liquid.
Guaiac testing is a method for detecting biological fluids (blood, gastric juice, vomit and feces) in special “police” systems or test strips. This method has a number of advantages over other methods, such as quality and speed of analysis.
The method is based on the use of guaiac test, which is a chemical compound that, when reacted with acids, alkalis and alcohols, can form a dark blue dye. Blood and other body fluids contain iron and other elements that can also form a blue dye when exposed to hydrogen peroxide and an alcohol solution of guaiac. If the reagent hits a surface where there are blood pigments, a blue tint appears. This blue color is an indicator of the presence of blood.
An important aspect when using guaiac samples to determine the presence of blood is its accuracy. The method is quite accurate due to the fact that biological fluid not only causes the appearance of blue color, but also changes its intensity and color. At the same time, it should be noted that the method has its drawbacks. It may be a false positive if, under the influence of the reaction, the guaiac paint loses its blue color and becomes colorless. False positive results can also occur in cases where a given