Middlebrook-Dubeau Reaction

The Middlebrook-Dubos reaction is a biochemical test that is used to determine the activity of bacterial flora in samples of biological material.

This reaction was developed in the 1930s by American bacteriologists G. Middlebrook and R. Dubos. It is based on the use of special nutrient media containing certain components that stimulate the growth and reproduction of bacteria.

When carrying out the middlebrock reaction, samples of biological material (for example, blood, urine, sputum, etc.) are placed in special Petri dishes containing a nutrient medium. The plates are then incubated for a certain time at a certain temperature.

As a result of the reaction, the bacterial flora present in the sample multiplies. This produces bacterial metabolic products that can be detected in the sample after incubation.

The Middlebroke-Dubeau reaction assay can determine the number and type of bacteria present in a sample, as well as their activity. This can be useful in diagnosing various diseases associated with bacterial infection.

However, it should be noted that the Middle Brooke-Dubeau reaction cannot be used as the only diagnostic method. It should be supplemented by other methods, such as microscopy, culture on nutrient media, etc.

The Middlebrook-Dubos reaction is a method for determining the content of free carbon dioxide (CO2) in carbon dioxide (CO), which is one of the most important indicators of the quality of oxygenated drinks, such as mineral water. The method allows you to determine the amount of carbon dioxide contained in carbon dioxide. However, despite the fact that this method is widely used in industry and medicine, it is necessary to take into account some nuances in the application of the Middlebroke-Dubos reaction.

The name of the method comes from the names of two scientists - chemists G. Middlebrick and R. Dubos, who described the reaction in 1882. During the study, scientists observed the formation of a red solution. They later showed that the intensity of the solution's color is proportional to the amount of CO2 in oxygen. This discovery helped create one of the most common and reliable methods for determining CO2 concentration. The reaction was named after two great French scientists. It was discovered in 1840 and comes from sodium carbonate