Nenetsky-Sieber Test

The Nenetsky-Sieber test is a method for determining the protein content in the blood, which was developed at the beginning of the 20th century by domestic biochemists M.V. Nenetsky and N.O. Sieber. This method is widely used in clinical practice for diagnosing various diseases associated with protein metabolism disorders.

The principle of the method is that when a special reagent (copper sulfate) is added to a blood sample, a complex is formed, which turns red. The intensity of the color is proportional to the amount of protein in the sample.

The Nenetsky-Sieber test is one of the most accurate and reliable methods for determining the protein content in blood serum. It allows you to quickly and accurately determine protein concentration, which is especially important in the diagnosis of many diseases, such as nephrotic syndrome, hepatitis, liver cirrhosis, heart failure and others.

However, like any other method, the Nenzko-Sieber test has its limitations. For example, it may give false positive results if there is a large amount of bilirubin or other substances in the blood that can interact with the reagent. Also, this method is not suitable for determining protein content in patients with bleeding disorders or hemolysis.

In general, the Nenets-Sieber test remains one of the most reliable and accurate methods for determining the protein content in the blood and is widely used in clinical practice.

NENETSKY-ZIBER TEST - a method for determining the protein content in biological media and feed. Developed by the Soviet biochemist M. V. Nenetsky and livestock specialist N. O. Ziber. The first patent application for the proposed method is dated 1926 and put into practice in the mid-30s. Advantages of N.-Z. over old methods - accuracy, high reproducibility, fast work completion, ability to perform in low light. It is based on the formation of substances based on proteins that have the properties of sedimentary salts. The thicker the broth, the less sediment there will be. To carry out the test, the well-mixed broth is neutralized, then shaken again to prevent the emulsion from separating, put in a dark place for several hours, after the time has elapsed, the degree of alcohol concentration is determined with methyl alcohol, and the dry residue with silver nitrate. The color of the sediment is used to judge the total nitrogen content in the analyzed material. Protein appears in the second decade of the appearance of the white zone after neutralization, earlier than 2 decades with an ash mass fraction of more than 12%. There is a dark blue color of the sol and solution and the appearance of a characteristic odor of ammonia, which resembles the smell