Pappenheim Coloring

Pappenheim staining is a staining technique used in microscopy to identify microorganisms in samples. It was developed in 1928 by German scientist Otto Pappenheim and named after him.

Pappenheim staining occurs by applying a dye to the sample that binds to the cell wall of microorganisms. The sample is then treated with an acid, which destroys the cells, leaving only the colored proteins. The sample is then washed and dried and the colored microorganisms can be seen.

This method is used to detect various microorganisms including bacteria, fungi and viruses. It is one of the most common staining methods used in microbiology and medicine.