Test with Lugol's Solution

A coagulation test is a method for diagnosing infectious diseases, based on identifying the presence of antibodies to a specific pathogen in the patient’s blood. One of the most common methods of coagulation test is a test with Lugol's solution, which allows you to detect the presence of antibodies in the blood serum.

To carry out a test with Lugol's solution, a special solution containing iodine and sulfuric acid is used. When this solution is added to blood serum, a reaction occurs that results in the formation of antibody complexes with iodine. If antibodies to a specific pathogen are present in the serum, then when Lugol's solution is added, they will bind to iodine and form stable complexes. These complexes will be visible as dark spots on a white background, which allows you to determine the presence of antibodies and, accordingly, the diagnosis.

A test with Lugol's solution has a number of advantages over other methods for diagnosing infectious diseases. Firstly, it is fast and easy to carry out. Secondly, it allows the detection of antibodies to a wide range of pathogens, including viruses, bacteria and fungi. Thirdly, it makes it possible to detect even weakly expressed antibodies, which makes it especially useful in diagnosing chronic infections.

However, despite all the advantages, the test with Lugol's solution is not a universal diagnostic method. It can give false-positive results in the presence of other antibodies in the serum, as well as if the procedure is performed incorrectly. Therefore, it is recommended to use this method only in combination with other diagnostic methods, such as PCR or ELISA.

In general, the Lugol's solution test is an important tool in the diagnosis of infectious diseases and can be useful in detecting antibodies to various pathogens. However, it is necessary to take into account the limitations of this method and use it only in combination with other diagnostic methods.

A test with Lugol's solution is a coagulation test in which Lugol's solution is used to obtain the result. The test method belongs to the group of chemical analyzes using the precipitation method, and is called the Lugolevsky test. This analytical method was developed by French chemist Maurice Ledegro in 1907. The test is carried out to determine certain substances in biological material (spleen, liver, blood plasma, serum, etc.).

To carry out the Lugol test, a concentrated solution of iodine and a solution of sulfuric acid are required. It is possible to use powdered zinc oxide in small quantities so that iodine cannot settle on the walls of the test tube. Then acetic acid is added to a small amount of biological fluid. When all components are added, you need to mix thoroughly. To enhance the effect, it is recommended to bring the mixture to a boil. Next, Lugol's solution should be added to the sample after settling the samples. It is enough to wait until the sediment settles from the bottom of the glass. The precipitate must be carefully removed and added to another test tube with kainic acid. The result of the test will be noticeable in half an hour. The result is assessed by viewing the tube under a microscope. If the result is positive, then the sediment contains protein-carbohydrate complexes that are easily stained with iodine. If the test is negative, it means that the protein structures do not have complexes with other substances.

A test with Lugol's solution is one of the most accurate methods for studying human biomaterials. However, the reaction results may be inaccurate, especially if the samples were obtained from patients with metabolic or endocrine disorders, or in cases where the fluid contained anticoagulants. In addition, sample results may vary depending on the timing of the analysis.