Wilson-Blair Wednesday

Wilson-Blair medium (W.J. Wilson, 1879–1954, bacteriologist, E.M. Blair) is a medium for culturing bacteria that was developed in 1912 by William John Wilson and Edward Maclean Blair. This medium is an analogue of Agar medium with the addition of ferrous sulfate, which allows better visualization of bacterial colonies.

Wilson-Blair medium was named after its creators, who used it to study bacteria that cause disease in humans and animals. This medium is used for cultivating various types of bacteria such as Staphylococcus aureus, Escherichia coli, Salmonella typhi and others.

The main components of Wilson-Blair medium are peptone, agar and ferrous sulfate. Peptone is a source of nutrients for bacteria, and agar is the basis of the medium. Ferrous sulfate gives the medium a characteristic color that allows bacterial colonies on the medium to be easily visualized.

One advantage of Wilson-Blair medium is that it can be used for long-term storage of bacteria, which can be useful in bacteriology research. In addition, this medium can be used to determine the sensitivity of bacteria to antibiotics, which is important for choosing effective treatment for diseases caused by bacteria.

However, like any other bacterial culture medium, Wilson-Blair medium has its drawbacks. For example, it may not be very suitable for handling small bacteria such as E. coli, which may not grow well on this medium.

Wilson's medium is a mineralized nutrient medium used in microbiology and clinical bacteriological research for the isolation and cultivation of bacteria. Based on food hydrolyzate of animal origin, to which salts of calcium, magnesium, potassium, sodium, iron, phosphorus, lemon are added