Vinogradsky Wednesday

The article “Vinogradsky Environment” will discuss the concept of “Vinogradsky Environment” and its use in microbiology.

Vinogradsky Wednesday (also known as Vinogradsky Wednesday) is a special nutrient medium developed by domestic microbiologist Sergei Nikolaevich Vinogradsky. The medium was created in the 1880s and was used to study microorganisms that live in nature.

One of the main advantages of Winogradsky Media is its ability to support the growth of various types of microorganisms, including bacteria, fungi and viruses. This allows researchers to study their biological processes and functions in conditions that are as close to natural as possible.

In addition, Vinogradsky Medium is highly stable and allows the preservation of microorganisms for a long time. This makes it indispensable for storing microbial samples and conducting long-term studies.

Today, Winogradsky Medium continues to be used in microbiological practice and is one of the most popular nutrient media in the world. It is used to create new drugs, study environmental processes and develop methods for water purification.

Vinogradsky environment

Vinogradsky Sergei Nikolaevich (12.12. 1868 - 29.02.1938), microbiologist, professor, creator of one of the most popular media for cultivating microorganisms. Wednesday was named after him. Along with sugar and salt agars - representatives of the most famous cultures of microorganisms, Winogradsky medium deservedly belongs to the classics. The traditional framework was developed by Winogradsky in 1887 and first published in 1982 [4]. It was used to study the influence of methane bacteria on the gas composition of the atmosphere, as well as to study the activity of sulfate-reducing bacteria. Winogradsky's medium included acidic water, salt, ammonia and wheat seeds or other nutrient substrate. This environment did not retain methane, which created problems for studying the process of methane formation by bacteria [5,6]. In addition, this medium contained sucrose, which was toxic to microorganisms, and it was difficult to dilute them on this medium.

S.N. Winogradsky proposed using saline urine as a carrier [4], which avoids some of the disadvantages of Winogradsky’s usual medium. In addition, the addition of several reagents provides the formation of different growth substrates. Currently, Winogradsky’s medium is widely used to create nutrient mixtures used for the cultivation of natural microbial communities - pure cultures of rhizosphere and endomycrypha plants [7], detection and determination of pathogens of human, animal [8] and feed [9]. Based on Winogradsky’s medium, a liquid medium was created for the cultural study of sulfite-reducing bacteria spores [10].