Endrashikka-Cleghorn Method

The Jendrasik-Cleghorn method or E-K method is one of the most common methods for determining blood glucose levels. This method was developed in 1926 by Czech physician Ludwig Jendrasik and American biochemist Robert A. Cleghorn, and it is still used in medical practice to measure blood glucose levels in patients with diabetes and other metabolic diseases.

The principle of the E-K method is that glucose contained in the blood reacts with Jendrasik's reagent, which is a solution of ferric chloride and acetic acid. In this case, a complex compound is formed, which turns blue. The color intensity is proportional to the concentration of glucose in the blood.

To carry out the analysis, you need to take a blood sample and add a solution of Jendrasik's reagent to it. Then the mixture is stirred and left for 5 minutes. After this, the color intensity of the solution is measured using a photometer. The test result is expressed in mg/dL (milligrams of glucose per deciliter of blood).

The E-K method has several advantages over other methods for determining blood glucose, such as the Friedman method or McLeod method. It is more accurate, faster and more economical, and does not require the use of special equipment. In addition, the E-K method is widely accepted and available in most medical laboratories around the world.

However, like any other method, the Jendraszik-Cleghon method has its limitations. For example, it is not suitable for patients with hemolysis (destruction of red blood cells) or high levels of bilirubin in the blood. This method may also give false positive results if there are other sugars in the blood, such as fructose or galactose.

Overall, the Jędrasik-Cleghorn method is an important tool for diagnosing and monitoring blood glucose levels and is widely used in medical practice.