Hematoxylin Delafilda

Delafield's Hematoxylin: features and application

Delafield's Hematoxylin is a dye mixture obtained by combining hematoxylin and eosin. It is one of the most common stains used in histology and cytology to identify nuclear components of tissues and cells.

History of creation

Delafield's hematoxylin was first described in 1867 by French chemist Jules Delafield. He combined hematoxylin, which stains nuclear components blue, and eosin, which stains the cytoplasm pink.

Application

Delafield's hematoxylin is one of the most commonly used stains in histology and cytology. It is widely used to stain tissues and cells to highlight nuclear components and determine tissue structure.

In addition, Delafield's hematoxylin can be used to isolate certain types of tissues and cells, such as metaplastic cells in endocervical specimens and cartilage tissue and bone tissue in skeletal muscle specimens.

Preparation

Delafield's hematoxylin is prepared by mixing hematoxylin and eosin in certain proportions. The following reagents are commonly used:

1. Harris Hematoxylin - 0.1 g
2. Eosin - 1 g
3. Ethyl alcohol - 100 ml
4. Ethanol - 100 ml
5. Glycerin solution - 100 ml
6. Acetic acid solution - 100 ml

First, hematoxylin is dissolved in ethanol, then the remaining reagents are added and dissolved until homogeneous.

conclusions

Delafield's Hematoxylin is an important tool in histology and cytology and is widely used for staining tissues and cells, identifying nuclear components, and determining tissue structure. It was developed in the 19th century and remains a popular dye to this day due to its reliability and versatility.