Histological Technique

Histological technique is one of the main methods for studying biological tissues and organs. It includes a set of methods used for the preparation and analysis of histological preparations, which make it possible to study the structure and function of tissues and organs at the microscopic level.

The main steps of the histological technique include:

1. Tissue or Organ Preparation: Tissues or organs must be thoroughly cleaned of blood, fat and other contaminants and cut into thin sections.
2. Dehydration: Sections of tissue or organ are treated with alcohol to remove residual fluid and preserve their structure.
3. Fixation: Sections are treated with formaldehyde or other fixing agents to keep tissues and organs intact.
4. Staining: sections are stained with special dyes to make them more contrasting and easier to examine under a microscope.
5. Microscopy: histological preparations are studied under a microscope, which allows you to see the structure of tissues and organs in great detail.

Histological techniques are widely used in medicine, biology and other scientific disciplines. It allows you to study various diseases such as cancer, infections and other pathological conditions, as well as study the normal processes of tissue and organ development.

Overall, histological techniques are an important tool for understanding biological processes and diseases, and their applications continue to expand in various fields of science and medicine.

Histological techniques are a set of methods and techniques used for the preparation and examination of histological preparations (tissue sections). Thanks to these techniques, we can study the microstructure of various tissues and organs at the cellular level, which allows us to understand the functions and mechanisms of their functioning. In other words, histological techniques provide information about how they structure, function and what changes occur in various tissues, organs and systems of the body.

To prepare a histological specimen of tissue, it is necessary to cut it into thin layers (no more than a few micrometers), which are called tissue sections or paraffin blocks. These sections are then mounted on glass measuring 2.5 x 7.5 cm in such a way that the least amount of unexplored areas remain on the glass. For this purpose, glasses can be coated with various reagents or dehydrogenated, as well as fixed in formaldehyde vapors or other reagents. These procedures are aimed at stabilizing the structure of tissues and preventing their unwanted changes under the influence of air and moisture. After this, the paraffin blocks are poured into paraffin and “polished” until a transparent, uniform, parallel surface of the block and the entire plane of the preparation are obtained, onto which the contrast agent is applied.