Glucose Oxidase Method

Glucose oxidase method: Determination of glucose content in biological fluids

In medical diagnostics and laboratory practice, the glucose oxidase method is widely used to determine the glucose content in blood and urine. This method is based on the oxidation reaction of glucose in the presence of the enzyme glucose oxidase, which leads to the formation of hydrogen peroxide. In turn, hydrogen peroxide is oxidized with the participation of peroxidase, which leads to the formation of colored products. The amount of colored products formed is proportional to the concentration of glucose in the sample, allowing one to judge its level.

The operating principle of the glucose oxidase method is based on the interaction of glucose with the enzyme glucose oxidase. Glucose oxidase catalyzes the oxidation of glucose, converting it to gluconolactone and forming hydrogen peroxide. The next step is the oxidation of hydrogen peroxide with the participation of peroxidase, which leads to the coloring of the reaction mixture.

One of the important components of the glucose oxidase method is the enzyme glucose oxidase, which specifically interacts with glucose. This enzyme is a biological catalyst and is widely used in biochemical analyzers and test systems for determining glucose content.

The glucose oxidase method procedure includes several steps. First, a blood or urine sample is mixed with a reagent containing glucose oxidase and peroxidase. Glucose oxidase then reacts with glucose in the sample, resulting in the formation of hydrogen peroxide. After this, hydrogen peroxide is oxidized by peroxidase, which leads to the formation of colored products. The intensity of the color is proportional to the concentration of glucose in the sample.

The glucose oxidase method has several advantages. First, it provides high specificity and sensitivity, which allows you to accurately determine the glucose content in blood and urine. Secondly, this method is fast and easy to perform, which makes it convenient for use in clinical practice. In addition, the glucose oxidase method has a wide dynamic range, which allows the determination of both low and high glucose concentrations.

However, some limitations of the glucose oxidase method should be taken into account. Certain substances or conditions may interfere with test results, including the presence of ascorbic acid, hemoglobin, or medications. Also, sample preservation and storage may affect glucose stability and measurement accuracy.

In conclusion, the glucose oxidase method is an effective and widely used method for determining glucose levels in blood and urine. It is based on the oxidation reaction of glucose in the presence of the enzyme glucose oxidase, which leads to the formation of hydrogen peroxide and colored products. This method is highly specific, sensitive and easy to perform, making it a useful tool in clinical diagnosis and medical research. However, it is necessary to take into account possible limitations and factors that may affect the accuracy of the results.