Kaskelo Method

Kaskelo Method: spectrophotometric method for the quantitative determination of coproporphyrin in urine

The Kaskelo Method is a spectrophotometric method that is used to quantify the level of coproporphyrin in urine. Coproporphyrins are a group of pigments that are formed during the decomposition of hemoglobin. Changes in the level of coproporphyrin in urine can serve as a sign of various diseases, such as porphyrinopathies, hemolytic anemia and other disorders of porphyrin metabolism.

Kaskelo Method is based on the use of zinc chloride to oxidize coproporphyrin and form porphyrin. The porphyrin then reacts with the diazonium salt to form a red product that has a maximum absorbance at 554 nm. Measuring the absorption of this product allows the amount of coproporphyrin in the urine to be determined.

The Kaskelo Method has high accuracy and sensitivity, which makes it a useful tool for diagnosing porphyrinopathies and other diseases associated with impaired porphyrin metabolism. However, the method has some limitations, such as the possibility of influencing the results of the analysis of certain drugs and the duration of the study.

Thus, the Kaskelo Method is an important tool for the quantitative determination of coproporphyrin in urine and can be used to diagnose various diseases associated with impaired porphyrin metabolism. However, its limitations must be taken into account and only used in combination with other diagnostic methods.

In medicine and biology, coproporphyrins are a group of porphyrins that are formed during iron metabolism. Coproporphyrin is one of the main components of the oxidation of bile acids and hemosiderin. It is often used as an indicator of the state of iron metabolism, when assessing the iron content in the blood.

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