Necklaces Test

The necklace is a method for identifying anthrax bacilli. It is based on the fact that sticks grown on agar break up into balls, which are arranged in chains, forming a necklace. This method is used to identify the causative agent of anthrax and other diseases caused by bacteria.

The necklace method was developed in 1926 by American bacteriologist John E. Snow. He used this method to identify bacteria that cause disease in humans. The necklace method has become one of the most popular bacterial identification methods in the world.

To perform the necklace test, bacteria must be grown on an agar plate supplemented with penicillin. Penicillin is an antibiotic that kills most bacteria except anthrax. When the sticks grow on the plate, they begin to break up into balls. These balls are arranged in chains and form a necklace.

The necklace test is a simple and quick method for identifying anthrax bacilli. However, it is not 100% accurate, as some bacteria may not form into balls or form chains. Therefore, to confirm the diagnosis, the necklace test must be supplemented with other methods.

Overall, the necklace test remains one of the most common methods for identifying anthrax bacilli to this day. It is easy to use and allows you to quickly identify the causative agent of the disease.

Necklace: a unique identification test for Bacillus anthracis

Anthrax is a dangerous disease that can be fatal if not diagnosed and treated promptly. Although there are effective treatments and preventions for this infection, it is important to recognize it early to ensure maximum benefit from treatment and prevent the spread of the disease. One method for identifying this dangerous pathogen is the necklace test.

The necklace test is a method for identifying anthrax bacteria in tissue samples or biological material using a special dye, methylene blue, which penetrates the bacilli cells and stains them a characteristic blue color. Depending on the method of execution, the test can be sensitive and effective even after repeatedly freezing and thawing the sample. It can also be used to detect even very small colonies of microorganisms. In some cases, this method can be used to determine the bacterial contamination of environmental objects, but is not suitable for testing food products.

Theoretically, when performing the necklace test, you can get the same result as using standard bacterial cultures. In practice, in order to create bacterial colonies on a nutrient medium, a certain amount of time is required for the colonies to grow, dry, and color in a certain color (the bacteriophage secreted by the test rod can provoke coloring in