Coloring Background

Background staining is one of the methods of staining histological preparations, which is used to create a background against which the elements of the structure under study are more clearly distinguished. A histological specimen is a specially prepared tissue sample that is used to study the structure of tissues and organs under a microscope.

Background coloring can be done in various ways, depending on the purposes of the study. One of the most common methods is hematoxylin and eosin (HE) staining. With this method, hematoxylin stains the cell nuclei blue, and eosin stains the cytoplasm and extracellular matrix pink. Thus, the background is painted in a light blue or pink color, which makes the elements of the structure under study more noticeable and expressive.

In addition, background coloring can be performed using other coloring substances, such as azure II, methylene blue and others. Each staining method has its advantages and disadvantages, and the choice of method depends on the purpose of the study.

Background staining is an important step in histological studies, as it improves the visibility of tissue elements and facilitates their identification. Without coloration, the background tissue may appear uniform and difficult to analyze. However, due to the coloring of the background structures, the tissues become more distinguishable and stand out from the background of the colored structures.

In conclusion, background staining is an important method for staining histological specimens to improve the visibility of tissue elements and facilitate their identification. This method has many applications in scientific and medical research, and is also used for educational purposes to train medical and biological students.



Background coloring is an important and integral part of the histological technique for studying tissues, organs and structures of the body. The role of staining is to create a background against which the structure of the tissues being examined can be examined in detail. Before applying solutions to fabric, it is necessary to give it the appropriate structure, color and transparency. This is done using various tools and techniques such as sanding, chemical treatment, and background painting. Thus, coloring the backgrounds allows for the correct identification of tissue elements, which is an important element in their study.

What is background coloring? Background staining is one step in the process of processing and examining biomedical samples using a microscope. This process involves obtaining accurate information about the structure of the tissue or organ in which the study is located. Using certain chemical solutions and instruments, different areas of the sample are stained and this creates not only contrast between different areas, but also between cells. The separated parts of the material under study become contrasting. This helps researchers better see and study details in tissues and organs.

Why is background coloring necessary? Background staining is an important part of the tissue examination process. Thanks to it, it is possible to improve visual contrasts between different zones of the material under study and improve the quality of microscopic