Otan-Method

Otan method

The Otane method (also known as the osmium tetroxide-α-naphthylamine method) is a histochemical method used to visualize and identify nerve cells.

This method is based on the fact that osmium reacts with unsaturated lipids present in the myelin sheaths of nerve fibers and the cell membranes of nerve cells. When α-naphthylamine is added, a black precipitate forms on the surface of the nerve structures, allowing them to be visualized under a microscope.

The Otan method is often used in neurobiology to study the morphology of the nervous system, assess the degree of myelination of nerve fibers, and also to identify changes in nervous tissue under various pathological conditions. The advantage of this method is the speed and ease of staining, as well as the good contrast of the resulting preparations.

Otan method is a method for determining the concentration of hydrogen ions in aqueous solutions, which is based on the reaction between hydrogen ions and naphthylamine, which results in the formation of a colored complex, the color intensity of which depends on the concentration of hydrogen ions.

The Otan method was developed in 1960 and is used in various fields of science and technology, such as chemistry, biology, medicine, etc. This method is simple, accurate and fast, making it one of the most popular methods for determining the concentration of hydrogen ions in water.

To carry out analysis using the Otane method, it is necessary to add a small amount of naphthylan to the solution and observe the change in color of the solution. The intensity of the color depends on the concentration of hydrogen ions, which allows you to determine their concentration.

The advantages of the Otan method include simplicity, accuracy and speed of analysis, as well as the ability to be used for various types of solutions. However, like any other method, it has its disadvantages, such as the need to use special reagents and equipment, as well as the impossibility of application in some cases due to the specific properties of the solutions being analyzed.