Lymphocyte Blastotransformation Reaction

Study of the cell blastotransformation reaction in immunology

The blastotransformation reaction of lymphocytes is one of the most important methods for studying the body’s immune system. This method is used to diagnose allergic reactions, assess the state of the immune system and identify its disorders. The method is based on the ability of lymphocytes to actively transform in response to the addition of a nonspecific stimulator to their cells. The intensity of this process can be used to determine the effectiveness of treatment and the state of the immune status.

Lymphocytes (cells of the immune system) have the ability to actively divide and transform into mature immune cells. At the moment of tissue infiltration by a foreign substance, the cellular immune system triggers a cascade of reactions to destroy the foreign agents. One of these reactions is the reaction of blastotransformation of lymphocytes. It is a process in which lymphocytes spontaneously transform into blasts, that is, they become highly differentiated and capable of division. Activation of the process occurs under the influence of nonspecific or specific stimulants. In this case, increased cell proliferation is formed, as a result of which the level of biologically active substances increases and, as a result, various symptoms of the disease appear. However, in healthy people, this process is not active, which determines the use of blastotransformation in medical practice.

As part of the diagnosis of allergic diseases, the blast cell transformation reaction is applicable both at the laboratory and clinical levels. For experimental purposes, blood serum is used after injection to test allergens, which makes it possible to determine the presence and titer of sensitized

The blastotransformation reaction of lymphocytes is a diagnostic method in immunology. The amount of blastotransformation can be maximum or minimum in the majority of individuals examined. Maximum blastotransformation of lymphokine-induced cryoareminal lymphocytes (number of active cells) can be observed in more than 20 thousand cells under factor conditions