Rnf

RSF: what is it and why is it needed?

If you're interested in microbiology, you've probably heard of phage titer rise reaction (PHRT). But what is RSTF and how is it related to the concept of "RNF"? In this article we will look at this term in more detail.

Rnf is an abbreviation for “phage titer rise reaction.” RSTF is a method for measuring the number of phages in a bacterial culture. Phages are viruses that infect bacteria. RSTF is used to determine the concentration of phages in a solution or to evaluate the effectiveness of phage therapy.

The principle of RSTF is that viruses can only infect bacteria in certain quantities. If there are few viruses in the solution, then they will not be able to infect all bacteria, and their number will not change. But if there are enough viruses in the solution, they infect all the bacteria, and their number increases. Thus, it is possible to determine the number of phages in a solution based on changes in the number of bacteria.

To carry out RSTF, it is necessary to make a series of serial dilutions of the original bacterial culture and add the same number of phages to each diluted culture. The number of bacteria in each culture is then measured after a certain period of time after incubation. Measurements are carried out by sowing crops on solid nutrient media or using photometry.

RSTF has many applications, including studying the interaction of viruses and bacteria, determining the effectiveness of phage therapy, and studying the mechanisms of bacterial resistance to phages.

In conclusion, it can be said that RSTP is an important method for studying the interaction between viruses and bacteria. The use of this method makes it possible to more accurately determine the number of phages in a bacterial culture and evaluate the effectiveness of phage therapy.