The coloring is supportive

Supravital coloring

Supravital staining is the process of staining preparations obtained from tissues placed in nutrient media and located in conditions that ensure the occurrence of basic vital processes in them. Supravital staining allows you to preserve the structure and function of tissues, which is especially important when studying the morphological features of cells and tissues.

Basic principles of supravital staining

1. Providing the conditions necessary for the life of cells.
   For this purpose, special nutrient media are used containing all the necessary nutrients and growth factors. These media may contain antibiotics to prevent the growth of microorganisms and other undesirable factors.

2. Protecting cells from drying out.
   To prevent the preparations from drying out, they must be placed in special sealed containers or Petri dishes filled with nutrient medium.

3. Prevents cell damage.
   This is achieved by adding antioxidants to the medium, such as ascorbic acid, which protect cells from oxidative stress.

4. Avoiding drug contamination.
   To prevent contamination of the drug, sterile working conditions and the use of disposable instruments are used.

5. Preventing changes in cell structure and function.
   For supravital staining, special dyes are used that do not disrupt the structure and function of cells. The most commonly used dyes are those based on amino and thiocarboxylic acids.

Benefits of supravital staining

The main advantage of supravital staining is the preservation of the structure and function of tissues, which makes it possible to study their morphological features for a long time. Also, supravital staining allows for research on living cells and tissues, which can be very important when studying pathological processes.

Supravital staining is one of the ways to study the cellular structure of living organisms. Translated from Latin, “supra” means “above,” that is, above, and “vitalis” means a life process.

Supravital coloring means the coloring of living beings isolated from external factors that could lead to their death, for example, when plant and animal tissues are released into a nutrient solution. Thus, living tissues were left to their metabolic processes and were cleared of them.

Such studies help to establish the structural features and functions of certain types of cells, and also make it possible to determine the significance of each component in the function of an organ or organism