Bach-Zubkov Method

The Bach-Zubkov method is a protein analysis method developed by Soviet biochemists Anatoly Bakh and Svetlana Zubkova in the 1930s. The method is based on the use of chromatography and electrophoresis to separate proteins into their individual components.

The Bach-Zubkova method includes several stages:

1. Separation of proteins into fractions by gel electrophoresis. This allows proteins to be separated into several fractions based on their size and charge.
2. Identification of proteins by comparing their amino acid sequence with known proteins. For this purpose, methods of mass spectrometry and peptide analysis are used.
3. Determination of the concentration of each protein in the mixture. For this purpose, the spectrophotometric method is used.
4. Analysis of protein properties such as structure, function, interaction with other proteins, etc.

The Bach-Zubkov method is widely used in biochemistry and molecular biology to study proteins and their functions in living organisms. It is also used in medicine for the diagnosis and treatment of various diseases associated with protein metabolism disorders.

Bach-Zubkov method

**The Bach-Zubkov method** is an experimental scheme for the development of life based on the following hypotheses and observations of scientists over a long period. The main emphasis is on methods for studying the vital activity of organisms, on which their isolation, purification, etc. depend. These aspects were highlighted and disclosed back in the 60-70s of the 20th century. The Bach-Zubov method brings these theories to life through experiments on living organisms.

The history of the name of the method stretches from the Institute of Biology, whose director is Academician of the USSR Academy of Sciences A.N. Bach. According to him, this method is more effective when the vital activity of organisms is studied under conditions favorable for experiment. Sergey Zubkov studied this system, which proves the development of research in this direction.

The principle of the method is as follows: 1. Organisms adapt to certain environmental conditions up to a certain age. Changes in environmental factors cause restructuring of organisms. 2. These changes change the ability of animals to survive in changing conditions. 3. With any changes in conditions, adaptation of organisms must occur, otherwise they will not be able to adapt to such changes. Throughout the entire training in the theory of resistance to environmental factors familiar to them, animals acquire the ability to adapt to their environment: animals learn to change on their own. 4. The result of adaptation appears immediately after the change in environmental factors begins (this technique is sometimes called the classical method).

It is possible to identify characteristic **signs of life according to this method**: * Nutrition In the process of feeding, the body releases waste in the form of gas, which is removed either by water through the gills or lungs, or by