Biondi-Heidenhain Painting

Biondi-Heidenhain staining is a tissue staining technique that was developed in the 19th century by the Italian pathologist Alfredo Biondi and the German anatomist Otto Heidenhain. This method has been used to study tissue structure and identify various diseases.

To dye fabrics, Biondi used a mixture of dyes, which made it possible to highlight different structures in the fabrics. One of the main components of this mixture was hematein, which bound to hemoglobin in the blood and made it possible to detect it in tissues.

The Biondi-Heidenhain staining method was very popular in the early 20th century and was used to study various diseases such as cancer, tuberculosis and other infectious diseases. However, with the development of new methods of dyeing fabrics, this method has become less popular.

Today, Biondi-Heidenhain dyeing is used only in some scientific research and museums for the preservation of fabrics.

Beketova K.A. 404. Biondi—Heidenhain coloring

Contents Biondi-Heidenhainoc staining (or simply bionding) is one of the most stable and easy-to-perform techniques for staining material for histological and pathomorphological studies. For biondation, alcohol solutions of the basic fuchsin dye (alizarin red, acidic hematein basic) and eosin are used. Biondi staining was first described by the Italian pathologist Alfonso Biondi in 1852 in his doctoral dissertation. Even earlier, the method was proposed by Henri Biron-Heidenhain, after whom this technique was named. As a rule, the material is prepared by impregnation, and then the material is double-contrasting with basic fuchsin and eosin using the Biondi method. When stained with alcohol, the chromatin turns red. As a result of morphological analysis using this technique, the presence of fibrous structures, small nuclei, tubules, granular component, membrane-like structures and other components becomes clear. An important feature of this technique is that the preparation remains transparent throughout the entire staining process. This feature can be used to highlight additional colored structures. The Bionda technique is sometimes performed in the form of impregnation.