The G-nadi-oxidase (or greffa-nadi) reaction is a method used in biochemistry to determine oxidase activity in tissues or cells. This method was developed in the 1950s and is used for the determination of various enzymes such as oxidase, catalase and peroxidase.
The method is based on the use of hemetin, a dye that binds to heme contained in oxidase enzymes. When a hemetin solution is added to a tissue or cell sample, it stains the oxidase enzymes, allowing their activity to be determined.
The G-nadi-oxidase reaction is widely used in biological and medical research, allowing the assessment of the amount and activity of enzymes in tissues and cells. In particular, this method is used to study oxidative stress associated with various diseases such as cancer, cardiovascular disease and diabetes.
In addition, the g-nadi-oxidase reaction can be used to determine the effectiveness of treatment of diseases associated with impaired enzyme function. For example, in cancer treatment, where chemotherapy can cause cell damage, this can be detected using the g-nadi-oxidase reaction.
Thus, the g-nadi-oxidase reaction is an important method in biochemistry and medicine, which makes it possible to study the functions of enzymes and determine the effectiveness of treatment of various diseases.