Haemocytometer

Haemocytometer - what is it and how is it used in medicine and science

A hemocytometer, also known as a Neukammer chamber, is one of the most common instruments in medicine and science for counting cells in the blood. It was invented in the 19th century by the German hematologist Ernst von Neukammer and has since become an integral part of laboratory diagnostics.

A hemocytometer is a special glass chamber of a predetermined volume, which has two chambers located parallel to each other and separated by a thin partition. The cameras look like a rectangle, which is divided into small squares. The chamber volume is 0.1 µl.

The use of a hemocytometer begins with a drop of dissolved blood being applied to the surface of the chamber. The chamber is then covered with a glass plate, which distributes the blood evenly throughout the chamber. The chamber is then placed under a microscope and the cells in the chamber begin to be counted.

Visually, using a microscope, the number of different cells present in the blood sample being examined is counted. The number of cells is counted in certain areas of the chamber, and then the average number of cells per unit volume is calculated. This method of counting cells is called the "hand counting" method.

However, hemocytometers have now been largely replaced by electronic cell counters. Electronic cell counters have higher counting accuracy and speed than hemocytometers and can process a larger sample volume.

However, the hemocytometer is still an important tool in medicine and science, especially in cases where a more accurate blood test or a small sample volume is required.

Thus, hemocytometer is an important tool for counting cells in the blood and is widely used in medicine and science. Although electronic cell counters are now replacing hemocytometers in many applications, the hemocytometer remains an important tool for the accurate analysis of small blood samples.

The hemocytometric method is one of the methods for counting blood cells. It is used to estimate the number of different types of cells in a person's blood. Hemocytometry is based on the use of a special glass device called a hemocytometer.

Hemocytometers are used in clinical practice to determine the number of red blood cells, white blood cells and platelets in a patient's blood. They can also be used to determine hemoglobin levels and other parameters related to the number of blood cells.

To perform hemocytometry, a blood sample is placed in a special chamber, which is previously known for its volume. Using a microscope, the number of different types of cells that are present in the sample can then be visually counted.

One of the main advantages of the hemocytometric method is its accuracy and repeatability. In addition, it can analyze large numbers of blood samples quickly and efficiently, making it particularly useful for clinical research and diagnosing blood disorders.

However, currently, hemocytometric methods are gradually being replaced by electronic cell counters, as they are more accurate and process data faster. However, hemocytometry is still an important blood test and can be used in some cases to provide additional information about a patient's condition.

Hemocytometers are special glass chambers of a predetermined volume (known volume), into which pre-prepared and dissolved **blood is placed.** Then the **number** of different blood cells that are contained in the blood samples being studied are visually (using microscopy) counted and their qualitative properties are assessed.