Immunofluorescence

Immunofluorescence is a method for determining the amount and/or distribution of any antibody or antigen in a tissue section. In this process, antibodies are labeled (directly or indirectly) with a fluorescent dye (for example, fluorescein) and then exposed to tissue, a section of which is examined using an ultraviolet microscope.

In the case of direct immunofluorescence, the antibody is labeled immediately before it is exposed to the tissue.

With indirect immunofluorescence, the antibody is labeled after it is combined with the antigen, using fluorescent-labeling anti-immunoglobulin serum.

Immunofluorescent - related to immunofluorescence.

Immunofluorescence is a method for determining the amount and/or distribution of any antibody or antigen in a tissue section. In this process, antibodies are labeled (directly or indirectly) with a fluorescent dye (for example, fluorescein) and then exposed to tissue, a section of which is examined using an ultraviolet microscope. In the case of direct immunofluorescence, the antibody is labeled immediately before it is exposed to the tissue. With indirect immunofluorescence, the antibody is labeled after it is combined with the antigen, using fluorescent-labeling anti-immunoglobulin serum. - Immunofluorescent.

Immunofluorescence is a method for detecting and determining the concentration and localization of an antibody or antigenic substance in tissue sections. This process involves the use of a fluorescent dye and a specific antibody to bind to the antigenic surface.

The direct immunofluorescence method allows the fluorescent dye to directly bind to the antibody before it comes into contact with the tissue. For example, this can be used in determining the localization of proteins by applying a specific fluor marker protein to the tissue of interest. The use of direct methods allows us to obtain more detailed information about the biological process,

Immunofluorescence is one of the methods of special staining of preparations, which makes it possible to detect the presence of a particular protein (antigen) in the material being studied. This staining is based on the Ag-At reaction (antigen-antibody) or ABE (alternatively - antibody-antibody)