Immunohistochemistry

Immunohistochemistry (IHC) is a modern method used in medicine and biology to identify and localize various molecules on tissue samples by staining with special antibodies.

The method is based on the presence of colony-forming particles (colonizers, which are fragments of proteins and polysaccharides of microorganisms attached to the cell wall) in most types of pathogenic microorganisms. During immunization therapy, the synthesis of antibodies against these microorganisms increases, and the antibodies themselves are fixed on various cellular formations located inside the tissues of the body.

During research, an analysis is carried out for the presence and determination of the amount of antibodies of a certain class in certain areas of the tissue, differentiating between certain areas of the body tissue. The method is a classic example of the “method for recognizing related structures.” The main advantage of the method is the ability to increase the contrast of tissue images by pre-treating it with an anti-IgG antibody; as a result, it usually becomes much easier to evaluate the results of the study. However, recently experts have given preference to the use of antibodies specific to the cells being studied, for example, microbiology