Immunophoresis is a method of identifying an unknown antigen or detecting the presence of an antibody in blood serum. It is based on the occurrence of a precipitation reaction between an antibody and an antigen.
During immunophoresis, the antibody and antigen diffuse toward each other in an agar gel. When they meet, a precipitate line is formed. The location and shape of the precipitate line allows identification of the antigen or antibody.
Immunophoresis is widely used to detect abnormal proteins in serum in various diseases. It helps determine the presence of monoclonal immunoglobulins in myeloma and other gammopathies. This method is also used to identify bacterial antigens.
Immunophoresis is a method of identifying an unknown antigen or detecting the presence of an antibody in blood serum. This method is based on the occurrence of a precipitation reaction between antibody and antigen in an agar gel, which is a matrix for separating these molecules.
In immunophoresis, the patient's blood serum is placed in an agar gel that contains an antigen or antibody. The gel is then placed on a glass plate, which is coated with an antibody that binds to the antigen. After this, the plate is placed in electrophoresis, where the antigen and antibody begin to diffuse towards each other. When contact is reached, they begin to form a complex and are visually displayed on the glass plate in the form of stripes.
This method is widely used in medicine to diagnose various diseases such as HIV, hepatitis, syphilis, etc. It can also be used to detect the presence of antibodies to various infectious diseases such as tuberculosis, malaria and others.
One of the advantages of immunophoresis is its high sensitivity and specificity. This method allows you to determine the presence of antibodies or antigens even in very small quantities. In addition, immunophoresis is a fast and convenient method that can be performed in a laboratory setting.
However, like any other diagnostic method, immunophoresis has its limitations. For example, it may not be effective in the presence of large amounts of antigens or antibodies, or in the presence of inhibitors that may interfere with the reaction.
In conclusion, immunophoresis is an important diagnostic method in medicine that can identify unknown antigens and detect the presence of antibodies in the blood serum of patients. This method is widely used to diagnose various infectious diseases and other pathologies. However, like any other method, immunophoresis is not ideal and has its limitations that must be taken into account when using it.
Immunophoresis is a technique for laboratory testing of a patient’s blood serum, which can be used to identify pathological antibodies in the human body. This diagnostic method was created by French scientists in the middle of the last century. The result was the development