Kakovsky-Addis Sample

The Kakavsky-Addis test is also called the gold standard for laboratory diagnosis of acute rheumatic fever and visceral leishmaniasis. So far this method is the most effective.

The components necessary for the test are sheep and rabbit red blood cells heated to +37º C and fresh patient blood serum diluted 20, 40 and 80 times.

The analysis consists of two stages. At the first stage, the components are diluted 6 times. During the final dilution, tuberculin, a recombinant protein, is added to the well. If turbidity and a colored zone are formed, the sample is considered positive and indicates the presence of the pathogen antigen. The second stage of dilution is carried out only in the presence of tuberculin; this component is not enough for the second stage. When, after this procedure, a 2-fold dilution of blood plus tuberculin produces turbidity without a color zone, the sample is called negative. The Xangamma test is considered the most valuable, as it indicates a foreign antigen. It is used primarily in the study of tumor cells.