Light Color

Leka staining is a method for identifying Mycobacterium tuberculosis in smears and histological preparations by staining them with malachite green and safranin.

When carrying out Lek staining, smears or tissue sections are first treated with a solution of malachite green, which stains the mycobacteria bright green. Then the preparation is treated with a solution of safranin, which gives the background a pink-red color.

Thus, Mycobacterium tuberculosis stands out against the general background of the drug due to its bright green color, which allows them to be clearly identified under microscopy. Compared to other staining methods, Leka staining has high specificity for Mycobacterium tuberculosis.

This method is widely used in clinical diagnostic laboratories for quick and reliable detection of the tuberculosis pathogen in the test material.

Staining with malachite green with safranin (MGS) is a procedure for conservative staining of smears of cultures of gram-negative and gram-positive bacteria, used for species identification of microorganisms. The main painting process lasts about 30 minutes at room temperature. This process is performed only for pathogenic bacteria from the family