Myeloperoxidase

Myeloperoxidase: role in the diagnosis of leukemia

Myeloperoxidase (M) is the general name for enzymes of a subclass of peroxidases found in myeloid blood cells. It plays an important role in the differential diagnosis of leukemia and other blood diseases.

Myeloperoxidase is a key enzyme in the body responsible for the disposal of bacteria and other infectious agents. It promotes the production of reactive oxygen species, which are involved in the destruction of bacteria and other microorganisms. In addition, Myeloperoxidase plays an important role in the formation of bonds between proteins and cross-reactions between proteins and lipids.

Myeloperoxidase is also used in histochemical determination, for example, in the differential diagnosis of leukemia. This is because certain types of leukemia exhibit high Myeloperoxidase activity, while others exhibit no activity. Thus, determining the level of Myeloperoxidase can help in determining the type of leukemia and choosing the optimal treatment.

Recent studies have also shown that myeloperoxidase may be important in the development of various diseases such as atherosclerosis, bronchial asthma and diabetes. For example, activation of Myeloperoxidase can lead to the formation of free radicals, which can contribute to damage to the vascular wall and the development of atherosclerosis.

Thus, Myeloperoxidase is an important enzyme that plays an important role in protecting the body from infections and can be used in the diagnosis of leukemia and other blood diseases. Further research in this area may lead to new methods of treating and preventing various diseases.

The myeloperoxidase reaction belongs to the histochemical group of redox reactions for the study of primary amines, aldehydes and ketones, classes of organic compounds detected by histochemical methods at the cellular level. The enzyme myeloperoxidase is found in leukocytes and granulocytes of various animals (particularly mammals), including humans, and is used to diagnose certain malignant neoplasms. The first reliable laboratory method for assessing MP in peripheral blood is the Govart-Zikhov technique, which relates to visual reactions. The technique received its name from the authors who proposed it in 1928. Initially, the technique was intended to study the content of MP. The technique makes it possible to differentiate hypersegmented forms of neutrophils and SHANTS. . Red cell seeding method. In such cultures, in response to injury to myelocytes (immature cells), purple rosette-shaped spots appear after 5-7 hours of growth in a nutrient medium. The reaction is based on the enzymatic interaction of MP with hydrogen peroxide introduced into the substrate. Microscopically, a structural restructuring associated with the presence of maturing myelocytes after injury from germinal stem cells located in the bone marrow is revealed - the absence of globules in the cytoplasm of mature neutrophils.