Mobitza Interfering Dissociation

Mobitz interference separation

Mobitz - interference analysis

(English: Mobitz—Interference Dissociation (MID)) is a new approach associated with the analysis of DNA sequence fragments, which uses the so-called “meristic model” (from the English “mer” - “reading unit”) based on various pairwise displacement mechanisms bases in the process of genome unwinding.

The main idea of ​​Interfering Separation is to split the read content into a number of identical sequences (fragments) that can be analyzed independently or separately from each other, which can significantly increase the efficiency of the analysis and achieve greater sensitivity for detecting all changes in DNA sequences, wherever they occur. were not there.

The method was discovered in the first half of the 80s of the XX century. John Nemesh discovered that if we stretched the same DNA double-stranded region several times and analyzed that region each time using multiple laser light sources, we would obtain a unique set of fragments, among which all possible fragments would be present. including short ones (no more than about 25 base pairs) or very long ones (up to 300 bases or more), from which the original DNA sequence can be restored.

Based on all that has been said above, summarizing the entire procedure of the Mobile Interfering DNA Separation method comes down to the following: