Peroxidase Reaction Benzidine

Peroxidase reactions are one of the most common analytical methods in biology and medicine. Peroxidase is used to detect various substances such as enzymes, hormones and toxins. One of the most widely used methods is the benzidine peroxidase reaction (P. r. b.).

To carry out P. r. b. it is necessary to use a mixture of hydrogen peroxide (H2O2) and benzidine. When these two substances interact, peroxidase is formed, an enzyme that can change the color of the reaction mixture. Depending on the type of peroxidase and reaction conditions, the mixture may turn blue or brown.

When carrying out P. r. b., the reagents are applied to the sample, which may be biological material such as blood, urine or tissue. After incubating the sample with reagents, the color of the reaction mixture is visually assessed for the presence of peroxidase granules. If granules are present, this indicates the presence of the peroxidase enzyme in the sample.

Etc. b. has a number of advantages over other analysis methods. It is easy to carry out and does not require expensive equipment. In addition, it allows you to quickly and accurately determine the presence of peroxidase granules in a sample, which can be useful in diagnosing various diseases.

One of the disadvantages of P. r. b. is its sensitivity to reaction conditions. For example, changes in pH or temperature may affect the results. Also, there are some substances that can mask peroxidase granules, making their identification difficult.

In general, P. r. b. remains one of the most popular analytical methods in biochemistry and medicine due to its simplicity and accuracy. It allows you to quickly and easily determine the presence of various enzymes and toxins in biological samples, which is important for the diagnosis and treatment of various diseases.

Peroxidazine reaction - The benzidine test is used to detect Mycobacterium tuberculosis. It is based on the enzyme peridoxase, which is localized intracellularly. Using this method, the organism being examined is detected for the presence of tuberculosis bacteria, which are dangerous in their effects on surrounding people and animals.

To conduct the test, you need to take a sample of sputum from the throat or mouth. The process of determining contamination involves mixing a sample with a reagent. At certain stages of the study, the color of the material being tested may change. The color change is accompanied by precipitation and the appearance of stringy threads. These phenomena are signs of the presence of bacteria in the organism under study. If the result is positive, the patient will undergo further examination to establish a diagnosis. If a smear is obtained from the lip, then the positive result should not exceed 20%; for the mucous membrane of the larynx, this figure should be no more than 5%.