Phage Titer Increase Reaction

Phage titer rise test (PHRT) is a method used in microbiology to determine the concentration of bacteriophages in a solution.

The essence of the method is as follows:

1. Prepare a series of dilutions of the test solution containing phages.

2. A constant amount of bacteria sensitive to a given phage is added to each dilution.

3. The mixtures are incubated for a fixed time, sufficient for the phages to multiply.

4. After incubation, determine the smallest dilution of the solution at which bacterial lysis occurred (formation of lysis zones on the bacterial lawn).

5. Based on the smallest dilution that caused lysis, the phage titer is calculated - the number of viral particles in the original solution.

Thus, RSTF makes it possible to quantify the concentration of bacteriophages in a sample. This method is widely used in virology, microbiology and biotechnology.