Complement Fixation Reaction

The complement fixation test (CFT) is a laboratory test that is used to determine the presence of antibodies in the blood. Antibodies are specific proteins that are produced by the body's immune system in response to foreign antigens. RSK allows you to determine the presence of antibodies to certain infectious diseases, such as tuberculosis, syphilis, hepatitis, etc.

The principle of operation of RSC is based on the fact that when an antigen (infectious agent) is added to blood serum, an antigen-antibody complex is formed. This complex then binds to complement, a group of proteins that are involved in the body's immune response to infection.

To carry out RSK, a special test tube is used, into which antigen, blood serum and complement are added. The tube is then shaken and left for several hours or overnight. If antibodies are present in the serum, they will bind complement and color the solution red. If antibodies are absent, the solution will remain colorless.

RSK is one of the most common laboratory tests for diagnosing infectious diseases. It allows you to quickly and accurately determine the presence of antibodies in the blood, which can be useful in the treatment of infectious diseases and for preventing the spread of infections in society.

The complement fixation test is a laboratory method that is used to determine the presence of antibodies in blood serum or antigens on the surface of cells. This reaction is an important tool for diagnosing many diseases such as HIV, syphilis and hepatitis.

The reaction is based on the use of two different sera containing antibodies against the HIV virus (antibody) or hepatitis B antigen (antigen). The first serum contains an antibody that binds to an antigen and forms an antigen-antibody complex. The second serum contains complement, a protein that is activated in the presence of the antigen-antibody complex and begins to attack it. Thus, complement acts as a “prostitute” and fulfills its true purpose - to kill. The antibody binds to the surface of a cell containing an antigen (virus or hepatitis).

The appearance of a visible red clot in the tube indicates that the reaction was successful and a link was identified between the antigen in the sample and the antigen contained in one of the sera. If the reaction is not visible, this indicates a lack of binding or the presence of foreign substances in the sample.

The complement binding test is an accurate method that can be used to detect antibodies to various viruses, including HIV, hepatitis B and others. It can also be used to detect antigen on the surface of a cell.

Many studies have shown that HIV antibody levels may be elevated in patients with HIV infection, and high antibody concentrations may indicate the presence of infection. Therefore, the compliment binding reaction may be useful for diagnosing and monitoring the treatment of HIV infection. Besides,