Romanowsky-Giemsa Coloring

Romanowsky-Giemsa staining

Romanovsky-Giemsa staining is a method of staining blood smears and other biological preparations developed by the Russian physician Dmitry Leonidovich Romanovsky and the German chemist and bacteriologist Gustav Giemsa in the late 19th and early 20th centuries.

This method is based on the use of dyes from the azure-eosin group. When used, different elements of blood and tissue are painted in different colors, which allows them to be clearly distinguished under a microscope. Romanovsky-Giemsa staining is widely used for the analysis of blood smears, in particular for calculating the leukocyte formula, as well as for identifying pathogens of infectious diseases.

Synonyms for this method are: Giemsa staining, Romanovsky method. Romanovsky-Giemsa staining still remains one of the most common and informative methods for staining hematological and cytological preparations.