Shukevich Method

Shukevich Method: an effective way to isolate a pure culture of Proteus

In microbiology, there are many methods for isolating pure cultures of bacteria. One of the most effective and widely used methods is the Shukevich method. This method is based on the ability of Proteus to move across the surface of a slanted agar from the condensation liquid into which the material under study is seeded.

The Shukiewicz method was developed in 1892 by Polish microbiologist Albert Shukiewicz. It is one of the first methods for isolating pure cultures of bacteria. An important feature of this method is its simplicity and accessibility. It allows you to obtain a pure culture of bacteria without the use of complex equipment and special reagents.

Shukevich's method is based on the ability of Proteus to move across the surface of a slanted agar from the condensation liquid. To isolate a pure culture of Proteus, the test material, usually urine or other biological material, is placed in the condensation liquid. This liquid is then applied to the agar slant. In this case, the proteas begin to move along the surface of the agar and form colonies. Thus, each colony consists of only cells of one type of bacteria.

To obtain pure cultures of Proteus, it is necessary to isolate single colonies on agar. To do this, use a ball-shaped loop, which is heated to a red state and cooled to room temperature. Then, use a loop to take a small portion of the colony and apply it to a new agar slant. After this, the procedure is repeated several times until a pure Proteus culture is obtained.

The Shukevich method is one of the most effective and widely used methods for isolating pure bacterial cultures. It allows you to obtain pure cultures of Proteus without the use of complex equipment and special reagents. In addition, this method is low cost and can be used in laboratories with limited resources.

Thus, the Shukevich method is a simple and effective way to isolate pure cultures of Proteus. Its advantages include ease of use, availability and low cost. This method continues to be popular in microbiology laboratories around the world.

The Shukevich method is a method for isolating pure cultures of Proteus, based on their ability to move along the surfaces of slanted agar plates with the growth of bacterial colonies. In contrast to classical methods, such as the sequential introduction of a mixture of procaic acid with ammonium oxacetate using the Maurin sowing method, which leads to the production of several types of Proteas at once. Shukevich developed a method for isolating and identifying the most important representatives of Proteus with antimicrobial activity. He characterized certain types of bacteria as sensitive, moderately resistant and resistant to antibiotics, including peni