Tissue Culture Plasma

Plasma tissue culture is an in vitro method of culturing cells in which small pieces of tissue are placed in a clot of blood plasma.

Plasma contains many growth factors and nutrients necessary for cell survival outside the body. When plasma coagulates, it forms a gel in which pieces of tissue can be arranged in three-dimensional space. This allows you to preserve the natural structure of the tissue and interactions between cells.

To prepare a plasma clot, the blood is centrifuged, separating the plasma from the formed elements. Substances that initiate clotting, such as thrombin, are then added to the plasma. The resulting clot with pieces of tissue is placed in a nutrient medium and incubated in a CO2 incubator.

Plasma tissue culture is widely used in scientific research to study tissue physiology, drug testing, and in regenerative medicine. It allows you to maintain the viability of complex tissues such as the liver, spleen, and lungs for a long time.

Tissue culture is a culture method based on the ability of a piece of tissue to grow outside the body. The method originated in the 60s of the twentieth century, but its use began only in the 70s and 80s. It was developed primarily for plants and other multicellular organisms, but can also be used for culturing animal tissues. This article describes plasma tissue culture.

Plasma tissue culture is a technique that is used to propagate plants in a special nutrient solution through the propagation of plant parts (tissues) on a nutrient medium. To obtain a tissue culture, it is necessary to crush the plant (with a sharp knife or scalpel) into small pieces 0.5-1 cm in size and place them in plasma (a liquid obtained by passing castor oil through a heated, that is, water bath). After several days, gas bubbles forming from