Bogomolova Proba

The Bogomolov test is a qualitative test for urobilinogen in urine, based on the formation of a colored complex of urobilinogen with divalent copper ions. This method is widely used in clinical practice to diagnose diseases of the liver, biliary tract and other organs that can affect the production and excretion of urobilinogen from the body.

The principle of the method is that urobilinogen reacts with copper ions in the presence of sodium chloride, forming a complex that turns yellow. When concentrated sulfuric acid or dilute hydrochloric acid is added, the urobilinogen complex is decomposed into free urobilinogen and copper, which remains as a precipitate.

To carry out the test, urine is mixed with a solution of copper chloride and sodium chloride. Concentrated sulfuric acid or dilute hydrochloric acid is then added to break the complex. If urobilinogen is present in the urine, a yellow precipitate is formed, which can be seen visually or determined using a spectrophotometer.

Depending on the concentration of the urobilinogen complex, the test can be positive or negative. A positive result indicates the presence of urobilinogen in the urine and may indicate diseases of the liver, gallbladder, or other organs. A negative result indicates the absence of urobilinogen and can be used to monitor the treatment of diseases associated with its production.

The Bogomolov test has high sensitivity and specificity, and its results can be used to diagnose many diseases. However, like any other diagnostic method, it is not the only criterion for making a diagnosis and must be confirmed by other research methods.

Bogomolov (ortho-aminobenzenesulfophthalein) test is a qualitative test for the determination of urobilinogen in urine (a transition product of hemoglobin metabolism), based on a change in color with Bogomolov's indicator. Uracine or urobilin is a non-protein derivative of uric acid, which is formed in the body due to the metabolism of guanine and purine. The test is usually used to diagnose hemolytic anemia, hemoblastosis, and other diseases accompanied by increased levels of urobilin in the urine. To carry out the test, a urine sample is placed on a filter and Bogomolov's indicator is added, which forms a complex with urobilin. A change in the color of the indicator from purple to yellow indicates a positive test result