Brown Method

Brown John (1934-2014) - American pharmacologist and biochemist who developed a method for determining the activity of enzymes in biological systems. This method, named after him, is one of the most widely used methods in biochemistry and molecular biology.

Brown's method involves adding the enzyme to be determined to a solution of a substrate and measuring the rate at which the substrate is converted to a product. This rate depends on the activity of the enzyme and can be measured using special instruments such as spectrophotometers or electrophoretic systems.

One of the main advantages of the Brown method is its versatility. It can be used to determine the activity of various enzymes, regardless of their structure and function. This allows biochemists to study various processes in living organisms and develop new drugs.

However, Brown's method also has its limitations. For example, it cannot be used for enzymes that do not have or do not respond to a substrate. In addition, the accuracy of determining enzyme activity depends on many factors, such as substrate concentration, temperature and pH of the solution.

Despite this, Brown's method remains one of the most popular and widely used in biochemical research. Due to its simplicity and versatility, it allows you to quickly and accurately determine enzyme activity in various systems.

**Brown Method**

Named after John Harrington Brown, an American pharmacologist and biochemist who became the first university professor of pharmacology in the history of pharmacology (now the University of Illinois). He was the founder of the school and department of pharmacology at the University. Laval in Quebec and served as Dean of Pharmacological Sciences at the University of Alberta from 1971 to 1992. Brown was the first to demonstrate that β-adrenergic agonists (in particular, isoprenaline, a drug for the treatment of acute heart failure) stimulate human cardiac activity through their effect on the heart. This “railroad” effect makes this class of drugs the most effective in treating chronic obstructive pulmonary disease (COPD). Also, Brown noticed the existence of supraoptic and paraventricular adrenomedullin-producing cells of the hypothalamus - “secretory” neurons that secrete one of the main biological signaling molecules, hypothalamic gonadotropic hormone (GnRH), which reduces the function and secretion of sex steroid hormone precursors by the pituitary gland. This understanding is now of paramount importance because GnRH is a central player in the normal regulation of reproductive function in women and sperm proliferation in men. Under Brown's leadership, more than 250 scientific publications were carried out, which examined various aspects of the action of pharmacological and biochemical agents circulating in the body of mammals. However