Dfa-Proba

The DPA test (also Diphenylamine test) is an experimental method for the detection and determination of mutagens, based on the use of the diphenylamine reaction to the biochemical nature of the target substance, which also reveals the antimutagenic activity of this substance.

The method is based on a change in the intensity of diphenylamine red due to its ability to absorb or emit light, due to the transformation of pyrodinene into hyperodyne. The method is highly sensitive. It is often used as a detector of the action of various substances that affect the genetic material of a cell, and also as an indicator of the toxicity of these substances.

The DPA reaction is effective in determining chemical mutagenic factors, since the metabolism of these compounds produces the same products and they can be identified at the time of the reaction by their activity and therefore serve as a useful indicator of the effect of division products on cell cultures. In addition, with low solubility of sorbents, chromogenic effects on them are the most accurate indicators. The sensitivity of this reaction decreases after several applications of the sample. In general, the method has a number of advantages compared to other reactions: simplicity of the procedure, the ability to carry out without complex equipment, cost-effectiveness, high sensitivity and specificity.