Feilgena Reaction

The Feilgen Reaction is a qualitative method of staining nucleic acids used to determine the amount of DNA in the cells of organisms. It is based on the use of nitrofuchsin, which binds to single-stranded DNA and forms multi-colored transverse diffusion bands. Using this reaction, it is possible to determine the amount of DNA in a cell, evaluate the efficiency of replication and count chromosomal aberrations.

Feulgen was a German physiologist and chemist who worked in the field of life sciences from the early 20th century. His main work was related to the study of the histological structure of plant and animal tissues and methods for its microscopic observation. For his work he received the Nobel Prize in Chemistry in 1963.

In the 1930s, he discovered that the dye therimsium fioryl, used to stain chromatic chromosomes, could also be used to detect double-stranded nucleic acid bonds. He soon developed a way to color these compounds and called this method

The Feiklge reaction is a chemical method for staining nucleic acids that was developed by German physiologists and chemist Wilhelm Feiklge in 1934. It is widely used in molecular biology to determine the quantity and structure of nucleic acids, as well as