Frenkel Method

The Frenkel method is a method for isolating a blood culture of the causative agent of sepsis, which consists of inoculating the patient’s blood on a regenerated nutrient medium. This method was developed by the German scientist Ernst Fraenkel in 1931 and has since been widely used in clinical practice for the diagnosis and treatment of infectious diseases.

The Frenkel method is based on the use of two types of nutrient media - Tarozzi medium and McConkey medium. Tarozzi medium is a semi-solid nutrient medium that contains all the necessary nutrients for bacterial growth. After blood is inoculated onto such a medium, bacteria begin to grow and multiply, which makes it possible to isolate them from the blood.

MacConkey medium, in turn, consists of a nutrient medium containing blood and agar, which is a protective layer. After inoculation on this medium, bacteria that are in the blood begin to secrete toxins that can cause cell death. This allows pure cultures of bacteria to be isolated, which can then be identified and classified by microscopic examination.

One of the advantages of the Frenkel method is its high sensitivity and specificity. It can detect even the smallest amounts of bacteria in the blood, making it ideal for diagnosing sepsis. In addition, this method can be used to determine the sensitivity of bacteria to antibiotics, which helps to choose the most effective drug to treat the infection.

However, despite all the advantages of the Frenkel method, it is not universal and may not be applicable in some cases, for example, when there are a large number of other microorganisms in the blood or when there is insufficient blood for culture. In such cases, other methods of blood culture isolation may be used, for example, the blood agar method or the Endo medium culture method.

Thus, the Frenkel method remains one of the most effective methods for isolating a blood culture of the causative agent of sepsis and is widely used in clinical practice.

The Frenkel method for identifying pathogens in acute purulent-septic infections is one of the most effective and fastest methods. It involves inoculating the patient’s whole blood onto a special nutrient medium with the addition of components that allow bacteria to multiply and grow. This method allows you to identify and