Gomori S Method

The Gomori S Method is one of the most common methods for staining histological specimens. It is used to detect the presence of certain enzymes in samples, particularly phosphatase and lipase.

The Gomori method was developed in the 1940s by the American biochemist Gomori S. It is based on the use of special reagents that stain certain cell components light blue or purple. This method allows you to isolate certain structures in tissues, such as mitochondria, and determine the presence of enzymes in them.

The principle of the Gomori method is that it uses two main reagents: reagent A and reagent B. Reagent A contains copper sulfate, which turns tissue blue. Reagent B contains potassium hexacyanoferrate, which stains tissue purple. When using the Gomori method, the sample is first stained with reagent A, then with reagent B. This allows you to isolate certain structures in tissues and determine the presence of enzymes in them.

The Gomori method is widely used in medical practice to diagnose various diseases. For example, it can be used to determine the presence of enzymes in tumor cells. In addition, the Gomori method can be useful for studying mitochondria and other cell structures, which allows us to obtain more detailed information about the biological processes occurring in tissues.

Thus, the Gomori method is an important tool in histological studies and allows one to detect the presence of enzymes and determine cell structures with high accuracy. It is widely used in medicine and science and is an indispensable tool for studying biological processes in tissues.

Gomori's Method: Technique for staining histological samples to identify enzymes

In the field of histology, staining techniques play an important role in revealing the structural and functional features of tissues. One such method is the Gomori method, developed by the famous histologist Leslie Gomori. This staining method is widely used to detect the presence of certain enzymes in histological samples, especially phosphatase and lipase.

Staining of histological samples by the Gomori method is based on the interaction of enzymes with appropriate substrates and the formation of intensely colored reaction products. This method allows the identification and localization of enzyme activity in tissues, which is important for understanding and studying their role in biological processes.

The Gomori dyeing process includes several stages. First, the histological sample is fixed to preserve its structure and prevent tissue destruction. The sample is then treated with special reagents containing substrates for the target enzymes. Enzymes present in tissues interact with substrates and cause the formation of colored products.

One of the key advantages of the Gomori method is its ability to detect enzymes with high sensitivity and specificity. This allows researchers to pinpoint the location of enzyme activity in tissues and assess their levels. In addition, the Gomori method ensures the preservation of tissue structure, which allows further morphological studies.

However, it should be noted that Gomori's method has its limitations. First, it requires specific reagents, which can be expensive and difficult to obtain. In addition, determination of enzyme activity using this method requires experience and skill in interpreting stained samples, since the results may be influenced by factors other than enzyme activity.

In conclusion, the Gomori method is an effective and widely used method for staining histological specimens to detect enzyme activity, especially phosphatase and lipase. This method allows researchers to obtain information about the location and level of activity of enzymes in tissues, which is important for understanding their role in biological processes.

The Gomori method is one of the most common histological tissue staining methods used to study some important enzymes in the body such as phosphatase and lipase. This method was discovered by French scientist Louis Gomory in 1936 and named after him. The Gomori method is very sensitive and effective, especially when it comes to fast-acting enzymes. Directions for use: Prepare a 5% solution of HCl (a toxic substance) and sodium hydroxide in a 2% solution (cold). Heat this solution to a temperature of 70 degrees Celsius and add sulfuric acid (H2SO4), oxalic acid solution (HCOOH) and nitric acid. Heat the resulting mixture for another 5 minutes until the reaction passes. Then set the temperature for the nitrogen bath above the cuvette for