Goppe-Seyler Probe

The Hoppe-Seyler test is a method for determining the presence of bile pigments in biological fluids. It is named after the German physiologist and chemist Ernst Felix Joseph von Hoppe-Seyler (1825-1895).

The essence of the test is as follows: concentrated nitric acid is added to the test sample (urine, mucus, exudate) and causes the appearance of different colors depending on the presence of bile pigments.

If bilirubin is present, a green color appears (direct test). In the presence of biliverdin - blue-violet color (indirect test).

Thus, a positive Hoppe-Seyler test result indicates the presence of bile pigments in biological fluids and is an important diagnostic test for diseases of the liver and biliary tract.

The Hoppe-Seyler test is a method for determining the rate of diffusion of gases through porous materials. This method was developed in the 19th century by the German physicist and chemist E.F. J. Hoppe-Seyler (1825 - 1895).

The essence of the method is as follows: a gas mixture containing two gases is placed on the surface of the material, one of which has a high diffusion rate and the other a low one. The time required for the concentration of the first gas to reach a certain value over the entire surface of the material is then measured. This time depends on the rate of gas diffusion through the material and can be used to determine its porosity.

The Hoppe-Seyler test is widely used in various fields, such as chemistry, medicine, biology, etc. It allows you to determine the rate of diffusion of various substances through porous media, such as membranes, fabrics, etc.