Immunoelectrophoresis

Immunoelectrophoresis (IEF) is a method of electrophoretic diagnostics of biological objects, which consists in the separation of protein molecules (as well as polypeptides or peptides) using an electric field. In IEF, proteins are separated by binding them to specific antibody probes and then analyzed by detecting the probe protein with specific antibodies. The IEF algorithm includes the following stages: – application of the sample to a chromatographic membrane, divided into small chamber sections using carrier proteins (nucleic acids); – processing chambers to dissolve proteins; – incubation of chambers with immobilized probe molecules (microchip and camera scanning) that recognize the protein; – washing the remaining proteins and probe molecules from the substrate; – scanning the microchip and identifying point signals that are unique for each protein profile. Immunoelectrophoresis occurs according to the principle of electrophoresis