Digestion Method

The digestion method is a method for studying the structure of connective tissue, which is based on pre-treating a piece of tissue with enzymes to facilitate the visualization of cells and fibers. It is widely used in histology and biology to study various types of connective tissue.

The purpose of the digestion method is to improve the visibility of microscopic structures in tissues. Pre-treating the tissue pieces with enzymes changes the structure of the collagen fibers, making them easier to see under a microscope. This method also facilitates the identification and classification of different types of cells and fibers in tissue. As a result, it is possible to obtain more accurate and informative data on the state of connective tissue in the body, such as the condition of tendons, ligaments, skin and

The digestion method is a method for studying the structural organization of connective tissue using pre-treatment of pieces of tissue with digestive (protease) enzymes, which allows you to accurately determine whether cells belong to a specific tissue or cell type and assess the level of apoptosis. Thanks to this, it is possible to establish a number of physiological patterns of the existence and functioning of the structure of not only connective tissues, but also individual structural units of all tissues of living organisms. To do this, tissue fragments are pre-treated with gastrointestinal enzymes to obtain hydrolysates. Then the material is restored (by post-red autolysis), the autolysis products are filtered out, and the polymer model is fixed in the detergent-purified phase by studying