The Rosette method

The Rosette method is a method for detecting cells that carry specific antigen-binding proteins on their surface. This method takes advantage of the ability of these proteins to form mixed antigens or “rosettes” with corpuscular antigens such as red blood cells, bacteria or viruses.

The Rosette method was first described in 1951 by Robert Rosette and is one of the most common methods for detecting lymphocytes in the blood. It is used to determine the level of immune response in the body, as well as to detect infections, allergies and other diseases.

To carry out the Rosette method, special reagents that contain antigens are used. These reagents are added to blood samples and cause the formation of “rosettes,” agglutinated antigen-antibody complexes. The samples are then stained with special dyes that make these complexes visible.

Evaluation of the results of analysis using the Rosette method is carried out by counting the number of “rosettes” formed in blood samples. The more “rosettes” that are formed, the higher the level of immune response and the greater the likelihood of infection or disease.

In general, the Rosette method is a simple and effective way to determine the level of the body's immune response and identify various diseases, such as infections, allergies, cancer and others.

The rosette method is a method for detecting sensitized antigen by cells that have surface receptors when they are stained with a corpuscular antigen. It is based on the formation of a mixed agglutination of a sensitized cell with other corpuscular-stained cells. The method is designed for quick and convenient determination of sensitization to