Coloring Lifetime

Definition and significance of intravital coloring

Lifetime coloring (lat. color vitalis; synonym: life-coloring reagent) is the ability of some substances to paint an object in colors that differ from the color of the object painted with its “normal” natural color. The concept of staining with living dyes (LCD) includes all shades of saturated dyes, which differ in intensity and light absorption spectra in comparison with the natural color of the object under study. This also includes different degrees of color intensity, as well as its spectral composition, i.e., ultimately, the difference between one color and another. In earlier sources, the term “vitalization” was used. However, let us dwell on this term in more detail. This is a method of coloring objects in which the dye is used to identify not a dead, but a living object. Therefore, this method is called intravital staining. This method is used for painting muscles, internal organs of animals and humans. Staining with live dyes allows you to quickly and relatively easily identify wounds, tumors, cavities, blood clots, aneurysms and other pathological changes located in the “dead”, dry, sclerotic muscle, musculofascial or connective tissue material of the corpse and internal organs. The use of intravital staining makes it possible to perform the same work in less time, requiring physical effort and material support from medical personnel. Living dyes are special liquid preparations that produce color effects on the biopsy object. The peculiarity of such preparations is that the pathologically altered organ or tissue stained with them acquires a special, contrasting color compared to the normal object. This occurs due to the fact that specially selected dyes cause specific sensitive reactions from nerve cells through nerve endings in tissues, creating a sensation of color design. These reactions transmit information to the cerebral cortex, where, based on the analysis of the information received, the brain assesses the condition of the organ, makes a diagnosis and prescribes appropriate treatment. Thus, living dyes are not just a color mark applied to organs and tissues, but rather a factor that additionally influences the activity of the cell. And that is why the use of living dyes as diagnostic substances always remains a controversial process in the medical field. Some researchers believe that when used in conjunction with other forms of examination, many false signs of disease are detected. In addition, many practicing doctors often use dye preparations to diagnose complex they simply neglect other examinations, believing that they are not needed. As a result, the bottom line of everything suffers.