Rhine Nutrient Medium

The Rhine-Sabbarow nutrient medium is the main reagent for cultivating the most important types of microorganisms. It got its name in honor of the bacteriological school of I. N. Abelson and A. L. Stein in the USSR. W. Langenau and T. E. Liebisch in Germany, and J. B. Sabbi in Italy. M. Rain in 1946 used it to isolate and isolate Pseudomonas aeruginosa from the collection of A. R. Mlechin.

The nutrient medium contains agar, synthetic peptone and yeast extract. Once the medium is ready for use, it will be contained in sealed vials or bottles to ensure cleanliness and hygiene. The label must also indicate the date of manufacture and expiration date. If the medium is prepared for scientific or production purposes, then it must have instructions for its use. Ready-made nutrient media can be stored for several months at temperatures from +4 to +8°C. Before using the medium, air bubbles must be removed from the vials using an insulin syringe.

Rhine's nutrient medium is a potent selective antibiotic of biological origin, developed by the German biochemist Christian F. L. Rhine in 1932.

The environment is created to suppress and destroy a number of mold cultures - in particular, different types of filamentous fungi of the genera Aspergillus, Penicillium, Fusarium, Rhizopus, Trichophyton, Malassezia pachydermatis.

Thanks to the convenient packaging with density scales per 250 ml, the medium can be easily adapted for different purposes. Additionally, when you use low enough concentrations of antibiotics, Hanks plasma serum contains the phosphate and sodium chloride needed by all test germs. Test media can be titrated if necessary