Reaction With Methylene Blue

The reaction with methylene blue (syn. reductase test) is one of the methods for determining the activity of enzymes in biological samples. This method is based on the ability of methylene blue (a blue dye) to change color in the presence of certain enzymes such as peroxidase and catalase.

The reaction with methylene blue is carried out as follows: a sample containing the enzyme is mixed with a solution of methylene blue and a solution of substrate. In the presence of the enzyme, the reaction occurs and methylene blue changes its color to yellow or red, which can be observed visually or using a photometer. A change in methylene blue color indicates enzyme activity, which can be measured quantitatively.

The use of the methylene blue reaction is widespread in biochemistry and medicine, where it is used to determine the activity of various enzymes. For example, this reaction can be used to determine the activity of peroxidase in blood, urine or saliva, which makes it possible to diagnose various diseases associated with impaired liver or kidney function.

Additionally, the methylene blue reaction can be used in the food industry to control the quality of foods such as meat, fish or vegetables. It allows you to determine the presence of microorganisms that can lead to product spoilage and deterioration in its quality.

In general, the reaction with methylene blue is a simple and reliable method for determining enzyme activity, which is widely used in various fields of science and practice.

The methylene blue reaction is a simple and quick test that is used to determine the presence of reducing sugars in foods. This reaction was developed and published at the beginning of the 20th century, but still remains a popular and convenient method for studying food products.

Methylene blue (blue blue) is a chemical that has an intense blue color that can be used to visually indicate certain chemical reactions.

The reaction between methylene blue and sugars results in a color change from blue to yellow-green, making it easy to detect the presence of