Strain Tumor

Tumor strains To find tumor cells, extraction is used from soft tissues, organs, fluids (blood, urine, cerebrospinal fluid) or placental tissue of humans or animals. Tumor cells differ from normal somatic cells in their biological properties, i.e. main characteristics

A tumor disease of tissue that has been transplanted many times. A tissue strain can be characterized as having constant morphological and physiological properties.

- After a series of experimental transplantations, the rearranged, actively proliferating tumor grows again. - The morphological composition of cells in the body does not change and corresponds to the type of cells that form the basis of cellular

A strain of tumor tissue (lat. stamum tumoris) is tissue of transplantable tumors after transplantation into experimental animals with the presence of a number of morphological (infiltrating growth, extravascular and intracellular infiltration, accumulation of cells in the form of strands) and biological (sensitivity to narcotic sleep, inability to grow outside the body , the absence of chromosomal abnormalities in biopsies) properties that remain constant during repeated transplantations of tumor cells, differing from the properties of highly malignant cells of a sick person.

Tumor culture strains provide a unique way to study the molecular pathways of oncogenesis and can be used to develop innovative methods of diagnosis and therapy. Long-term culture of transplanted tumors also makes it possible to study various forms of the neoplastic process, including autonomous or mixed cell lines that differ in the level of expression of the transcriptome, metaphase chromosomes, glycoproteins and DNA components.

To clone tumor cells, neoplasms of the primary lesion are transplanted into contact with the immune system of the donor animal, being an ideal experimental material. Among the primary tumors, the most commonly used tumors are gliomas, soft tissue sarcomas, and breast carcinomas. The grafting method is used to study the effects of various factors (viruses

A tumor tissue strain is tissue of transplantable tumors after multiple transplantations into experimental animals and is characterized by constant morphological and sometimes phylogenetic similarity in all subsequent (regardless of experimental conditions) transplantations, and is a natural model for studying the morphological and biochemical (for example, metabolic) tumor homeostasis, constitutional and genetic variability of individual forms of animal tumors. Tumors of different organs of the same individual—donors in a state of “remission”—are studied as objects of a transplantation experiment. Laboratory technology for tumor cell transplantation is carried out in stages: obtaining monolayer cultures of tumor tissues, creating optimal conditions for their primary adhesion, transferring cells into a nutrient medium outside the animal’s body when preparing a tumor cell suspension.

The effectiveness of tumor therapy directly depends on the correct diagnosis of the disease, for which various methods are used, including clinical (history, physical examination), laboratory and instrumental. For these purposes, all kinds of biopsies can be used, including: immunohistochemical, XI (confocal laser scanning microscopy), PCR diagnostics.

Tumor cell strains are grown on special polymer nutrient media (DMEM, F-12 or McCoy, Theer's medium) in monolayers for 3-7 days at a temperature of 37C and a humidity of 5% For

A tumor strain is the tissue of transplantable tumors after multiple transplantations into experimental animals. It is characterized by the constancy of the physiological and morphological properties of these tumors. Thus, tumor strains are tumor tissues that have been repeatedly transplanted into animals causing tumor growth. This allows scientists to conduct research on various properties of a tumor without having to remove the tumor from the animal's body.

Tumor strains are important for cancer research. In recent years, many studies have been carried out to study the molecular mechanisms of tumor development and methods of their treatment. One of the main ways to create tumor strains is to transplant the tumor into experimental animals - the so-called “transplant” method. This method involves scientists removing a small part of a patient's tumor and implanting it into the animal's body, causing the tissue to begin to grow. Thanks to constant selection, the best options are selected from growing tumors to create tumor tissue strains. Tumor cultures make it possible to study the mechanisms of tumor transformation of both individual cells and multicellular structures - fibroblasts or epithelial tissues.